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KMID : 1024520030120080847
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Journal of the Environmental Sciences
2003 Volume.12 No. 8 p.847 ~ p.852
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Bacterial Die-Off in Continuous River Water Flow System
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Kong Surk-Key
Toshiuki Nakajima
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Abstract
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It was examined carefully that the bacterial die-off between Chlorella vulgaris and E. coli. W3110 was tested through adding TOC (total organic carbon) with the lab-scaled continuous river water flow system (CRWFS). Artificial synthetic wastewater was applied at two levels of organic carbon concentration; 1,335 mg/l in treatment type 1 and 267 mg/l in type 2. In both types, the population densities of Chlorella vulgaris were similar in a maximum 8.25 106/ cells/ml (type 1) and 6.925 106/ cells/ml (type 2). The maximum densities of E. coli. W3110 were 2.0 108/ colony forming unit (CFU)/ml in type 1 and 3.9 108/ CFU/ml in type 2. The densities increased for 11 days in type 1 and 4 days in type 2, then decreased rapidly till the 35th day, then slightly increased again. This trend was prominent in type 2. It implied that a wider range of nutrients was required in the growth of heterotrophic bacteria in type 2 than in type 1. We could not expect successful bacterial die-off if the wastewater retention time was not furnished sufficiently.
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KEYWORD
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Chlorella vulgaris, E. coli, W3110, survival pattern, wastewater retention time
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